Product Description
Chemical Name: (S)-2-(2-((S)-2-Acetamido-4-methylpentanamido)acetamido)-n-(4-methyl-2-oxo-2h-chromen-7-yl)-6-(2,2,2-trifluoroacetamido)hexanamide
Synonyms : 2S)-2-2-(2S)-2-acetamido-4-methylpentanoyl]amino]acetyl]amino]-N-(4-methyl-2-oxochromen-7-yl)-6-[(2,2,2-trifluoroacetyl)amino]hexanamide
(2S)-2-{2-[(2S)-2-acetamido-4-methylpentanamido]acetamido}-N-(4-methyl-2-oxo-2H-chromen-7-yl)-6-(2,2,2-trifluoroacetamido)hexanamide
Cas No : 1026295-98-6
Purity : 95%
MolecularFormula : C28H36F3N5O7
Fluorogenic Peptide Substrate for ResearchThis compound combines a peptide backbone with fluorinated and chromenyl groups, enhancing its utility in fluorescence-based assays. Researchers rely on its exceptional purity and stability for enzymatic studies, especially where sensitive detection is crucial. Its compatibility with DMSO, DMF, and certain aqueous buffers makes it versatile for varied biochemical protocols.
Safe Handling and Storage GuidanceTo maintain stability and activity, store the product at -20C, desiccated, and avoid repeated freeze-thaw cycles. It is non-poisonous under laboratory practice, yet always utilize gloves and protective clothing while handling. Protect from light to prevent photodegradation and ensure consistent performance.
Key Physical and Chemical CharacteristicsThis compound appears as a crystalline, white to off-white powder, is odorless, and chiral with two (S)-configuration centers. It demonstrates excellent purity (>98%) and solid stability up to a melting point of 150-158C, at which decomposition may begin. It dissolves readily in DMSO/DMF and partially in aqueous buffers, supporting various synthetic and analytical applications.
FAQ's of (S)-2-(2-((S)-2-Acetamido-4-methylpentanamido)acetamido) -n-(4-methyl-2-oxo-2h-chromen-7-yl)-6-(2,2,2-trifluoroacetamido)hexanamide:
Q: How should (S)-2-(2-((S)-2-Acetamido-4-methylpentanamido)acetamido)-N-(4-methyl-2-oxo-2H-chromen-7-yl)-6-(2,2,2-trifluoroacetamido)hexanamide be stored to maintain its stability?
A: The compound should be stored at -20C in a desiccated environment and protected from light. Avoid repeated freeze/thaw cycles to maximize shelf life and preserve its chemical integrity.
Q: What are the recommended applications for this fluorogenic peptide substrate?
A: This product is primarily used in biochemical research, including enzyme assays and fluorescent labeling, where its fluorogenic properties are especially valuable for monitoring enzymatic activity and molecular interactions.
Q: When is this compound typically employed in research settings?
A: It is typically used when sensitive detection of enzymatic reactions or protein labeling is needed, such as in activity-based screening or fluorescence-based quantitative assays.
Q: How do I dissolve this compound for laboratory assays?
A: The product can be dissolved in DMSO or DMF for optimal solubility. It is also partially soluble in some aqueous buffers, making it adaptable for a range of assay conditions.
Q: What benefits does the trifluoroacetamido and chromenyl fluorophore offer in this peptide substrate?
A: The trifluoroacetamido group imparts enhanced stability and bioactivity, while the chromenyl fluorophore provides strong fluorescence, enabling sensitive detection in assays and labeling experiments.
Q: Is this reagent safe to use in standard laboratory environments?
A: Yes, it is considered non-poisonous for laboratory use. Standard safety procedures, including the use of gloves and protective clothing, should be followed during handling.
Q: What is the shelf life and how long can I expect the product to remain active?
A: When stored under the recommended conditions (at -20C, desiccated, protected from light), the compound remains stable and active for at least 24 months.